Differences in ethidium bromide and 4'-6-diamidino-2-phenylindole staining profiles with regard to DNA fragmentation during apoptosis
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منابع مشابه
Rapid and simple method for double staining of bacteria with 4',6-diamidino-2-phenylindole and fluorescein isothiocyanate-labeled antibodies.
Bacteria were either heat fixed on microscope slides or filtered with 0.2 micron-pore-size Nuclepore filters. The samples were stained with 4',6-diamidino-2-phenylindole (DAPI) for total staining and with polyvalent rabbit antibodies and fluorescein isothiocyanate-conjugated swine anti-rabbit antibodies for specific staining. By switching between two different optical filter packages in the mic...
متن کاملFluorescence lifetime distributions of DNA-4',6-diamidino-2-phenylindole complex.
Time-resolved fluorescence of 4',6-diamidino-2-phenylindole (DAPI) complexes show that for a homogeneous polymer (polyd(AT) or polyd(A).polyd(T)) at high P/D (phosphate/dye) ratio, a single exponential component adequately describes the fluorescence decay. For the AT polymers at low P/D ratio or for native DNA, the decay cannot be described by a single-exponential term. A continuous distributio...
متن کاملBinding of 4',6-diamidino-2-phenylindole (DAPI) to AT regions of DNA: evidence for an allosteric conformational change.
The interaction of 4',6-diamidino-2-phenylindole (DAPI) with several double-helical poly- and oligonucleotides has been studied in solution using optical spectroscopic techniques: flow linear dichroism (LD), induced circular dichroism (CD), and fluorescence spectroscopy. In AT-rich sequences, where DAPI is preferentially bound, LD indicates that the molecule is edgewise inserted into the minor ...
متن کاملLocalization of DNA in Mature and Young Wheat Chloroplasts Using the Fluorescent Probe 4'-6-Diamidino-2-phenylindole.
The spatial organization of chloroplast DNA in developing and dividing wheat chloroplasts was studied in the light microscope using the fluorescent probe 4'-6-diamidino-2-phenylindole, which binds specifically to DNA.The DNA of wheat chloroplasts was localized at the periphery of the plastid, frequently in a discrete band. No relocalization of the DNA was observed during plastid replication. Th...
متن کاملIn Higher Plants by M E a N S of the Dna - Specific Probe 4 ' 6 - Diamidino - 2 - Phenylindole
The D N A in isola ted chloroplas ts was visual ized by the f luorescent p robe 4 '6 d i amid ino-2 -pheny l indo le ( D A P I ) . When exci ted with light of 360 nm, the D N A D A P I complex f luoresces bri l l iant ly at 450 rim. Nucle i also f luoresce but their nucleoli do not . RNase and Pronase t r ea tmen t of chloroplas ts did not affect the f luorescence but both preand pos t t r ea t...
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ژورنال
عنوان ژورنال: IUBMB Life
سال: 1998
ISSN: 1521-6543
DOI: 10.1080/15216549800204602